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In-Vitro Inhibitory Effect Of Different Extracts of Chaetomium Cupreum on Alpha Glucosidase, Alpha Amylase and Hepg2 Cancer Cells

Nazir Ahmad Wani, Sharmila Tirumale*

Objective: The present study investigates the effect of soil isolated fungus C. cupreum on alpha glucosidase, alpha amylase and HepG2 cancer cells.

Methods: The antidiabetic effect of C. cupreum extracts was determined by α-glucosidase and α-amylase inhibition assays and anticancer activity was done by MTT assay.

Results: The inhibitory effect on α-glucosidase enzyme by C. cupreum extracts was observed in ethyl acetate extract with the value of 57.88 ± 0.05, followed by n-butanol extract 21.27 ± 0.04, methanol extract 15.19 ± 0.05 and chloroform extract 12.34 ± 0.01 at 100 μg/ml. Whereas, the inhibitory effect on α-amylase enzyme by n-butanol extract with a value of 53.70 ± 0.10, followed by ethyl acetate extract 31.81 ± 0.05, methanol extract 20.51 ± 0.05 and chloroform extract 16.00 ± 0.05 at 100 μg/ ml concentration. In anticancer activity, n-butanol extract of C. cupreum showed significant cytotoxic effect against HepG2 cancer cells with IC50 value at 300 μg/ml.

Conclusion: Our study concludes that C. cupreum extracts possess potential phytochemicals that will be useful in antidiabetic and anticancer treatment after their purification and characterization