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Estimation of Total Phenol, Tannin, Alkaloid and Flavonoid in Hibiscus Tiliaceus Linn. Wood Extracts.

Vijay D Tambe, and Rajendra S Bhambar

The present study was performed to evaluate the total phenol, tannin, alkaloid and flavonoid contents in petroleum ether, ethyl acetate and methanol extracts of Hibiscus tiliaceus wood. Extraction of powdered wood material was carried out by continuous hot percolation method in soxhlet apparatus using petroleum ether, ethyl acetate and methanol as solvents. Gallic acid was used as standard for the determination of total phenol and tannin by Folin-ciocalteu method. Total alkaloid content was determined by chloride colorimetric method using quercetin as a standard. The results showed that ethyl acetate extract has high concentration of total phenol, tannin, alkaloid and flavonoid contents as compared by bromocresol green solution using atropine as a standard. Total flavonoid content was determined by aluminium to petroleum ether, ethyl acetate and methanol extracts. Ethyl acetate extract contained the total phenol of 30.18 and tannins of 83.03 as mg of gallic acid equivalents (GAE), alkaloids of 66.01 as mg of atropine equivalents (AE) and flavonoids of 91.01 as mg of quercetin equivalents (QE).