抽象的

Prevalence of Extended Spectrum �?²-Lactamase and AmpC �?²-Lactamase among Enterobacteriaceae and Pseudomonadaceae Isolated at Tertiary Care Set up in Tripura, India

Tapan Majumdar, Shibabrata Bhattacharya and Raunak Bir

β-lactam antibiotics are the most widely used chemotherapeutic agents. Commonest cause of resistance towards β-lactam antibiotics is the production of β-lactamases. Isolation, identification and antibiogram of the members of Enterobacteriaceae and Pseudomonaceae from the clinical samples. Detection of Extended spectrum β-Lactamase and AmpC β-Lactamase producing Enterobacteriaceae and Pseudomonadaceae by phenotypic method. Culture isolation, identification and antibiogram of the isolates were performed followed by detection of Extended spectrum β-Lactamase by Double Disc Synergy Test and Phenotypic disc confirmatory test, whereas AmpC β-Lactamase was detected by Disc Antagonism Test and Double Disc Synergy Test. Out of 200 isolates, 54% are Extended spectrum β-Lactamase producers, 48.5% are AmpC β-Lactamase producers. Predominant ESBLs producers are Klebsiella pneumonia and Escherichia coli. In case of Pseudomonadaceae, 88% of Pseudomonas aeruginosa and 100% of Pseudomonas fluorescens has shown presence of AmpC β-Lactamase and 66% of Providencia alcalifaciens produced combined Extended spectrum β-Lactamase and AmpC β-Lactamase. Out of the 97AmpC β-Lactamase producers, 45.4% and 54.6% are inducible and plasmid mediated AmpC β-Lactamase producers respectively. This study shows high rate of circulating extended spectrum β-Lactamase and AmpC β-Lactamase producers in our hospital setup. Development of antimicrobial stewardship program based on the local epidemiological data and national guidelines is the need of the hour.