抽象的

Methane Production Characteristics and Microbial Community Dynamics of Mono-Digestion Using Three Single Manures

Junnan Fang, Min Wang, Rongyu He, Taili Dong, Zhan Zhang, Yingcai Sun, Yuexiang Yuan, Dong Li, Shuixing Jia, Xiaofeng Liu, Zhiying Yan

This study investigated the methane production characteristics and the composition of microbial community during anaerobic monodigestion of three target substrates (chicken manure, pig manure and dairy manure) by semi-continuous digestion. The methane production of the dairy manure was only 0.49 L/d, while the chicken and pig manure was 2.24 L/d and 2.67 L/d, respectively. Barcoded pyrosequencing of the prokaryotic microbial 16S rRNA genes in the DNA showed that the active bacterial populations detected were those required for the hydrolysis of amended substrate, and the active archaeal populations belonged to methanogens of phylum Euryarchaeota, which were dominated by Methanosarcina. Principal coordinates analysis revealed that there were significant differences among the three single manures associated with prokaryotic communities, and the community structure of seed sludge was predominant in the adaptation of substrates. Overall, VFAs accumulation and methanogens reduction maybe lead to the decease of methane production of dairy manures.