抽象的

Irregular virulence genes expression of Vibrio parahaemolyticus in shrimp or seawater matrix

Aijing Zhao, Haiquan Liu, Wenshuo Sun, Qin Li, Yingjie Pan, Yong Zhao

The three virulence genes (tlh, tdh and trh) expression of five Vibrio parahaemolyticus strains (ATCC33847, ATCC17802, VP1, VP2 and VP3) in shrimp or seawater matrix at 9°C and 25°C were detected by the quantitative reverse transcription-PCR (qRT-PCR). The results showed that the transcription levels of tlh in ATCC17802 and VP3, tdh in VP1 were up-regulated, but the transcription levels of tlh and tdh in ATCC33847 were significantly (p < 0.05) down-regulated in shrimp samples compared with that in seawater samples. Additionally, lowtemperature had a positive effect on genes expression, including tlh and tdh in ATCC33847, trh in ATCC17802, tlh in VP2. Meanwhile, lowtemperature had a negative effect on the expression of tlh in ATCC17802 and tdh in VP1. Interestingly, the transcription levels of tdh in VP1 and tlh in VP3 cultured at 9°C and 25°C, respectively, were significantly (p < 0.05) higher in shrimp samples than that in other samples. These results indicated the expression of virulence genes in V. parahaemolyticus were irregular with the varying of environmental conditions.