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Anticancer Potential of Intestinal Mucus from Marine Catfish Tachysurus dussumieri on MDA-MB-231-Bio-Processing of Intestinal Mucus from Marine Catfish Tachysurus dussumieri on Human Breast Cancer Cell line

 Kavitha Raja, Lekshmi Bose, Livya Catherene Martin, Gracy Jenifer Sahayanathan, Deepakrajasekar Padmanaban and Arulvasu Chinnasamy*

 Fish mucus is rich in several bioactive substances and an exciting source of natural compounds with multidimensional properties. The present study investigates the anticancer effect of intestinal mucus of marine catfish Tachysurus dussumieri against the human breast cancer cell line (MDAMB-231). The biochemical analysis of crude intestinal mucus revealed that Protein (1.35 mg/ml), Lipid (1.086 mg/ml), and Carbohydrate (2.095 mg/ ml). The protein profiling via Native and SDS-PAGE have bands of both low and high molecular weight. The cell viability of MDA-MB-231 cells on treatment with crude intestinal mucus expressed an IC50 value at 800 μg/ml. Nuclear damage was observed by propidium iodide staining and the intensity of the membrane potential of mitochondria was investigated through Rh-123 staining. The incidence of DNA damage was further established by Agarose gel electrophoresis and the cell cycle arrest at the S phase was confirmed by way of Flow cytometry