抽象的

2015 ?????????????????? CRISPR ??????????????????????? - ??? - ????

周静文1,2、李江华1,2、吴军军1,2、杜国成1,2、陈剑1,2

????????????? A ??????????????????????????????????????????????????? (CRISPR) ???????????????????????????????? A?????? sgRNA ?????????????????? A ???? 223% ???????????????????????????????????????????? A ???????????????????? OD600 ???? 10%????????????????????????????? (2S)-??????????? A ???????????????????????????????????? (2S)-???????? 421.6 mg/L?????? (50.5 mg/L) ? 7.4 ????????????????????????????????????????????????????????????? A ???????????????????????????????????????????????????????????????????????????????????????????????? (pABA) ????????????????????????????????????????????????????????????????-3-?????? (trpC )?????????????? (aroH ) ??????????????????????????????? (pabAB ) ??????????? (pabC )????????????????? (pabABC ) ?????????????????????????????????????????????? pABA ??????????????????????????? (?????? pH ) ??????????????????????? 3.22 gl−1????????? 12.4% [C†mol/C†mol]????????????????????????? (ISRU)?????????????????????????????????????????????????????????????????????????? (BoPET???? Mylar®) ?????????????????????????????????????? BoPET ??????????????????????????????????? Kevlar® ??????????????????????????????????????????????????????????????????????????????????????????????????????????????? Bigelow Aerospace® ????????NASA?2017 ???????????????????????????????????????????????????????????????????? (ISRU) ??????????????????????????ISRU ???????????????????????Rothschild?2016 ????????????????????????????????????????????????????????????????????????????????Averesch ? Krömer?2018 ????????????????? (pABA) ???????????????——???????????????????????????????? POTABA®??pABA ????????????Farlow ? Krömer?2016 ??????? PET/Mylar® ??????????ABA ????????????? Kochi ? Hunsdiecker ??????????????????????? Kevlar® ?????????????????????ABA ?????????Morgan?1977 ????????????? Kevlar® ????????????????????? (Krömer ???2013) ???????????? pABA ???????????????????????????? 0.03 gl−1 (0.22 mM) ??????????????????????/????? 0.22 gl−1 (1.57 mM) (Averesch ???2016)????????????? pABA??????????????? 4.8 gl−1 (35 mM) ????Koma ???2014???????????????????????????????? 43.06 gl−1 (314 mM)?Kubota ???2016??????????????????????????????????????? pABA ???????????????????????????????????Nicholson ???2000 ??Horneck ???2010 ????????????pH ???????????????????????????????????????1987????????????????????????????????????????????????????????????Horneck?1993?? ?? ????2009????????????????????????????2009????????2011???????2014????????2012??2013??????????????????????????????????????????????????????????????L-??????????????Metabolic Engineering?Applied and Environment Microbiology???????52??????????Current Opinion in Biotechnology?Biotechnology Advances????????????????????????????????????????????????????Scientific Reports??Nature Press???Electronic Journal of Biotechnology??Elsevier Press????????06 gl−1 (314 mM) ????? (Kubota ???2016)?????????????????????????????????????? pABA ????????????????????????????????? (Nicholson ???2000?Horneck ???2010)??????????????????????????????pH ????????????????????????????????????????? (Horneck?1993)????? ???2009???????????2009???????????????????2011????????2014???????2012??2013??????????????????????????????????????????????????????????????L-??????????????Metabolic Engineering?Applied and Environment Microbiology??????52??????????Current Opinion in Biotechnology?Biotechnology Advances?????????????????????????????????????????????????????Scientific Reports?Nature?????Electronic Journal of Biotechnology?Elsevier???????06 gl−1 (314 mM) ????? (Kubota ???2016)?????????????????????????????????????? pABA ????????????????????????????????? (Nicholson ???2000?Horneck ???2010)??????????????????????????????pH ????????????????????????????????????????? (Horneck?1993)????? ???2009???????????2009???????????????????2011????????2014???????2012??2013??????????????????????????????????????????????????????????????L-??????????????Metabolic Engineering?Applied and Environment Microbiology??????52??????????Current Opinion in Biotechnology?Biotechnology Advances?????????????????????????????????????????????????????Scientific Reports?Nature?????Electronic Journal of Biotechnology?Elsevier??????????Current Opinion in Biotechnology???Biotechnology Advances????????????????????????????????????????????????????????Scientific Reports??Nature??????Electronic Journal of Biotechnology??Elsevier??????????Current Opinion in Biotechnology???Biotechnology Advances????????????????????????????????????????????????????????Scientific Reports??Nature??????Electronic Journal of Biotechnology??Elsevier???????   zhoujw1982@jiangnan.edu.cn