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STUDIES ON FERMENTATIVE PRODUCTION OF CITRIC ACID BY Aspergillus niger ISOLATE USING SORGHUM MALT AND IT��?S OPTIMIZATION

M.P.D.Prasad, V.Sridevi, N.V.Surendra babu , O.V.S Reddy, N.Harsha

Citric acid is a tri carboxylic organic acid is the most important acid produced by fermentation used in the food and pharmaceutical industries. The present work deals with the determination of optimum growth conditions for citric acid production by submerged fermentation by using strain of A.niger MTCC662 respectively. Isolation, screening and mutated the A.niger strain induced by UV irradiation, diethylsulphate and Co60 by providing different types of media and the submerged fermentation was carried out under various growth parameters like temperature, pH and media of Sorghum malt as substrate. Strain improvement studies for maximum production of citric acid obtained after the fermentation. Maximum yield was obtained from mutant type of A. niger MTCC 662 than wild strains were about 12.0g/100ml and 8.2g/100ml. It could be concluded that the A.niger MTCC662 mutant strains have produced high yield than wild strain of A.niger MTCC662 cultures.