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Purification and Characterization of Manganese Peroxidase from Musa Acuminata Stem and Its Effect on Degradation of Dye Effluent

Jeniffer Sylvia D, Jhansi Lakshmi V, Renuka Devi P

Various applications of Manganese Peroxidase such as transformation of dyes, biobleaching, biopulping, treating wastewater contaminated with phenols, chlorinated phenol & cresols enhances the interest for further study on the enzyme. The present aim of the study is to purify Manganese peroxidase from plant source Musa acuminata stem, an agro waste and to monitor its effect on effluent degradation. Manganese peroxidase was extracted from Musa acuminata stem juice using 0.1M Phosphate buffer, Ammonium Sulphate precipitation & Dialysis techniques were carried out to partially purify the enzyme. Column chromatography was packed with silica gel and chloroform with 0.1M Phosphate Buffer as elution buffer to achieve 10.8 fold purification with 32% enzyme yield, specific activity was found to be 24.5 U/mg. The Km and Vmax value with MnSO4 as variable substrate were calculated from MM and double reciprocal plots. The pH and temperature of the enzyme were optimized. The FTIR analysis of the degraded effluents was analyzed.