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Isolation, Preliminary Phytochemical and Antibacterial Activity Studies of the Constituents Present In Ethanol Extract Of Manjanathi Fruits

G.Valli & M.Murugalakshmi

Knowing the importance of Manjanathi fruit called as Indian Noni as extensively revealed by various scientific groups, our study focus on isolation of bioactive constituents of ethanol extract of Manjanathi fruit(EEMF) collected from Rajapalayam area of Virudhunagar district of Tamilnadu. Preliminary Phytochemical analysis revealed the presence of Alkaloids, Carbohydrates, Proteins, Amino acids, Phenols, Flavonoids, Glycosides, Anthraquinone glycosides, Coumarin glycosides, Oils and fats. Phenols ,Alkaloids,and Flavonoids were found to be present in moderately more in quantity. Agar well method was adopted for the determination of antibacterial activity, against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Pneumococcus pneumoniae, Klebsiella pneumoniae, Streptococcus Pyogens, Salmonella paratyphi , Citrobacter, Proteus vulgaris and Bacillus subtilis. . Compared to standard Ciproflaxcin, EEMF possessed higher activities against Streptococcus pyogenes (6mm) and Pseudomonas aeruginosa (10mm) and closer activity against Klebsiella pneumoniae(8mm) as that of the standard Ciprofloxacin. One Compound was separated from other constituents by column chromatographic technique using 60% Methyl acetate: 40% Chloroform solvent mixture, was found to have the molecular formula C9H18O.The compound was characterized by spectral techniques like Fourier Transform Infrared spectroscopy,Nuclear Magnetic Resonance spectra-1H NMR and 13C NMR. FT – IR Spectrum of isolated compound at 3394 cm-1 indicated presence of –OH group and band at 2975.96 cm-1 indicate the presence of (CH3- CH-) groupings. Absorption range from 1120 cm-1 -1030 cm-1 was due to (C-O) stretching. 1H NMR spectral studies revealed that a Singlet at 4.89 ppm is due to the -OH proton. A Multiplet at 3.69 ppm is due to the (-CH2- CH2-) grouping equivalent to 10 protons. A Septet at 1.233 ppm in due to presence of – CH – (CH3)2 group and a Doublet of 1.140 ppm due to CH3-CH- group. From the above datas , the compound was found to be 4-Isopropyl cyclohexanol.