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Antioxidant Potential of Coal Extracted Humic Acid on In-Vitro Propagation of Musa Accuminata: A Comparison Study with Humic Rooting and Keradix

S. Dhanapal, D. Sathish Sekar

Humic acid is a principal component of Humic substances, which are the major organic constituents of soil (humus), peat, coal, many upland streams, dystrophic lakes, and ocean water. It is produced by biodegradation of dead organic matter. Leonardite act as a best source for humic acid.The antioxidant activity of coal extracted humic acid (CHA) on in vitro propagated Musa accuminata was examined with commercially available Keradix (KHA), Humic Rooting (BHA). The Polyphenols was found to be higher in native plants of about 146.2μg/ml whereas in CHA it is 54μg/ml. Similarly the amount of protein in BHA is found to be 7μg/ml and CHA is 2.65μg/ml. The catalase activity was found to be higher in BHA is 0.09 U/mg compared to CHA which is estimated as 0.048 U/mg. GST was measured and it was found to be significantly increased in CHA of 582.99 U/mg compared with other commercially available products. The Ascorbic acid Peroxidase activity was higher in Keradix of about 725.31 U/mg APX. The SOD activity was inhibited higher in CHA of about 41.34%.