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A Review on High Performance Liquid Chromatography (HPLC)

Mukthi Thammana

Chromatography is defined as a set of techniques which is used for the separation of constituents in a mixture. This technique involves 2 phases stationary and mobile phases. The separation of constituents is based on the difference between partition coefficients of the two phases. The chromatography term is derived from the greek words namely chroma (colour) and graphein (to write). The chromatography is very popular technique and it is mostly used analytically. There are different types of chromatographic techniques namely Paper Chromatography, Gas Chromatography, Liquid Chromatography, Thin Layer Chromatography (TLC), Ion exchange Chromatography and lastly High Performance Liquid Chromatography (HPLC). This review mainly focuses on the HPLC technique its principle, types, instrumentation and applications.

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化学文摘社 (CAS)
谷歌学术
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研究圣经
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参考搜索
哈姆达大学
国际组织研究所 (I2OR)
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日内瓦医学教育与研究基金会
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