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A Review on Analytical Methods for Quantification of Amphotericin-B in Marketed Dosage Form and Biological Samples

 Chappa Bhagyasri*, Gowri Gollu, Ramreddy Godela

Background: Amphotericin-B is an antifungal antibiotic indicated to treat infections caused by leishmaniasis. After COVID-19 outbreak amphotericin-B was preferably used to treat black fungus caused by mucormycosis in people recovering from COVID-19.

Main text: To test purity, identity and quantity of amphotericin-B in dosage forms and biological samples always needs different analytical methods such as spectroscopic, chromatographic, electro analytical methods and micro biological assays. This review depicts several published analytical methods along with instrumental conditions for the detection and quantification of amphotericin-B during the last 20 years.

Conclusion: From literature reviews, HPLC with UV detection is majorly used for quantification of amphotericin-B in pharmaceutical formulations and LC-MS for biological samples.